Mixing Injectable 5-Amino-1MQ: Process & Organization
Since September, I've been running 5-amino-1mq as part of my protocol. I started with an [oral sublingual solution](https://retatrutidelog.com/posts/diy-5-amino-1mq-solution-trade-offs-lessons-learned), but the mouth staining got old. Switching to injectable was simpler - just raw powder, bacteriostatic water, and [filtering](https://retatrutidelog.com/posts/how-to-filter-peptides).
I just mixed a fresh batch at 50mg/mL concentration for my current 20mg daily dose. This post covers the mixing process and my labeling system for staying organized.
5-Amino-1MQ is a small molecule NNMT (nicotinamide N-methyltransferase) inhibitor. If you want the science on how it works, [this research paper](https://pmc.ncbi.nlm.nih.gov/articles/PMC5826726/) covers the mechanism and its effects on metabolism. I've documented my personal experience with it in my weekly check-ins.
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## Why 5-Amino-1MQ is Easy to Mix
If you've worked with other peptides, you know some are a pain to reconstitute. SLU-PP-332, for example, requires suspension and never fully dissolves - you end up with a cloudy solution that needs vigorous shaking before each use.
5-Amino-1MQ is the opposite. It's **highly water-soluble**, which means:
- No co-solvents required (just bacteriostatic water)
- Dissolves completely and quickly
- Results in a clear red solution
- Stays stable in solution without crashing out
- No shaking required before drawing doses
This makes it one of the most straightforward compounds to work with. If you can mix retatrutide or semaglutide, you can mix 5-amino-1mq.
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## My Current Batch & Protocol
I just mixed a new batch at 50mg/mL concentration:
**Concentration:** 50mg/mL
**Raw Powder:** 500mg
**Bacteriostatic Water:** 10mL
**Final Volume:** 10mL at 50mg/mL
**Current dosing:**
- 20mg daily (every morning, fasted)
- Just restarted after a 2-week break at the same 20mg dose I stopped at
- 0.4mL per dose at 50mg/mL
- 25 doses per vial = 25 days supply
The higher concentration means smaller injection volumes, which I prefer. A 20mg dose in 0.4mL vs 1mL makes a noticeable difference in injection comfort.
I labeled this batch with an expiration of 1/2026. That might seem aggressive, but 5-amino-1mq is a small molecule (not a fragile peptide chain), it's just reconstituted in bacteriostatic water, and I'm using sterile technique throughout. With proper refrigerated storage and sterile handling, it should remain stable well beyond the 25 days it takes me to use a vial.
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## The Mixing Process
The process is essentially identical to [filtering peptides](https://retatrutidelog.com/posts/how-to-filter-peptides), which I covered in detail in that post. Here's the quick version:
### Equipment Needed
- **[Raw 5-amino-1mq powder](https://amzn.to/4oAOp9n)** (500mg)
- **[Bacteriostatic water](https://peptidetest.com/products/pfizer-hospira-bacteriostatic-water-30ml-vial)** (10mL)
- **[0.22μm PES Sterile Syringe Filters](https://amzn.to/3KRUKPd)**
- **[10mL Sterile Empty Vials](https://amzn.to/434kuOH)**
- **[3ml Luer Lock Syringes](https://amzn.to/4haLG3Q)**
- **[Alcohol Prep Pads](https://amzn.to/4q8O3sh)**
### Step-by-Step
1.**Add bacteriostatic water to raw powder** - Measure 10mL of BAC water and add it to the 500mg of powder. It dissolves almost immediately.
2.**Clean vial tops** - Use an alcohol prep pad to wipe down the rubber stopper. Let it air dry.
3.**Draw the solution** - Use a syringe with needle to draw up all the reconstituted solution.
4.**Attach filter and fresh needle** - Remove the old needle, attach a 0.22μm syringe filter, then attach a fresh needle to the filter outlet.
**Why so many needles?** That first needle just went into a potentially contaminated vial. If you push that same needle through your expensive filter, you risk contaminating the filter membrane itself and defeating the entire purpose. Fresh needle = sterile filtering process.
5.**Filter into sterile vial** - Clean your sterile 10mL vial top with alcohol, add a vent needle, then slowly push the solution through the filter into the vial.
6.**Label and store** - Apply your label and store in the refrigerator.
The entire process takes about 10 minutes per vial.
---
## The Final Product
Here's what the finished solution looks like:
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Notice the distinct red color of the solution. This is normal for 5-amino-1mq - it has a characteristic red hue when dissolved. What you want to see is clarity with no cloudiness, particulates, or sediment. Just clean, filtered red solution ready for injection.
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## Labeling for Organization
When you're mixing multiple peptides at different concentrations, proper labeling isn't optional - it's essential. Grabbing the wrong vial could mean a massive dosing error.
I use a **[Niimbot B35s label printer](https://amzn.to/3L4WRzd)** for all my peptide labels. The 10mL vials give you more real estate than standard 3mL vials, so I designed a label with quick reference dosing information:

The label includes:
- Compound name (5-Amino-1MQ)
- Concentration (50mg/mL)
- Dosing reference table
- Date mixed
### Dosing Reference Table
The dosing reference on the label shows volume needed for different doses:
| Dose | Volume |
|------|--------|
| 10mg | 0.2mL |
| 15mg | 0.3mL |
| 20mg | 0.4mL |
| 25mg | 0.5mL |
This means I don't have to calculate anything when drawing doses - just reference the label and draw the correct volume.
### Use My Label Template
If you want to use this exact label design, copy the text below and import it into your Niimbot app:
> 5-amino-1mq 50mg/mL(50*30)】,After copying this information⊙68fa4769d72bcd5b98c285f1⊙,Open👉NIIMBOT👈。
Then open your Niimbot app and it will automatically import the label template.
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## Storage and Handling
**Storage:** Refrigerator (36-46°F)
**Shelf Life:** Labeled expiration of 1/2026 - stable for months when stored properly
**Light Exposure:** Keep in dark (vials in box in fridge)
Because 5-amino-1mq is a small molecule rather than a fragile peptide, it's more stable in solution than compounds like retatrutide or semaglutide. Combined with bacteriostatic water and sterile preparation, I'm comfortable with extended storage times. Each vial only lasts me 25 days anyway, so I'm always working with relatively fresh solutions.
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**Disclaimer:** The information provided on this site is for **educational and informational purposes only** and reflects my **personal experiences and opinions**. It is **not** intended as medical advice, diagnosis, or treatment. 5-Amino-1MQ is an **investigational compound** that is **not approved by the U.S. Food and Drug Administration (FDA)** for any medical use. Always consult a licensed healthcare professional before making any decisions regarding medications, supplements, or experimental compounds.
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